Keywords
fluoroscopy
osteoporosis
ionizing radiation
low doses
very low doses
genomic instability,
DNA double-strand breaks
γ-H2AX/53BP1 foci
Categories
How to Cite
Abstract
ABSTRACT
Objective: Osteoporotic vertebral fractures affect 1.4 million patients in the world annually. Symptomatic fractures are treated with minimally invasive fluoroscopically guided medical procedures and as result there is considerable amount of low dose irradiation registered for patients and staff. According to the Linear no tress-hold model (LNT) used for evaluating radiation cancer risk, no radiation dose is considered save.
Aim: Our aim was to determine the amount of DNA damage after low dose irradiation of patients with osteoporosis undergoing vertebroplasty procedure.
Methods: We used fluorescent microscope visualization of the immunochemical co-localization of γ-H2AX and 53BP1 DNA damage repair proteins, so-called DNA damage-repair foci (DRF), to quantify the amount of DSBs (double-strand breaks) in lymphocytes from peripheral. The analysis included 42 patients with within the age range of 60 ÷ 91 years old (71, mean) and who received mean cumulative air kerma dose 22.07 mGy (5÷86.1mGy, range). All doses received by the patients during fluoroscopy time were in the low dose range (≤100mGy).
Results: A total of 8,200 cells were analyzed. We found statistically significant increase in γ-H2AX/53BP1 DRF frequency in patients after vertebroplasty 1.28 ±0.06 compared to the initial frequency before the procedure 0.70 ±0.04 (p<0.0001). This rise in DRF wasn’t statistically correlated with the amount of cumulative air kerma dose received nor the age of the patients.
Conclusion: This preliminary study showed a significant DNA damage increase after minimally invasive vertebroplasty procedures despite the low and very low doses received during fluoroscopy time. Miss-repaired DNA DSBs have the potential to manifest in stable chromosomal aberrations.
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Copyright (c) 2024 I Ivanova, E Zaharieva, M Atanasova, D Georgieva, N Kostova, L Hadzhiyska, Kristian Ninov, H Hristov, A Staynova, Rositsa Petrova Hristova (Author)